There are many humans seen throughout the games, some which serve as major allies and enemies of Shantae.
- Shantae (possibly half-human, see Warrior's relationships)
- All inhabitants of Scuttle Town seen so far
- Relic Hunters
- Save Guy
- Possibly Tassel Town residents before it was destroyed
- Risky Boots (presumably)
- Twitch and Vinegar
- Some Ammo Baron's soldiers
- Holly Lingerbean (half-human half-genie, before becoming a memory)
- Tinkerbats could be mutated forms of humans who fell victim to The Pirate Master's magic before the main timeline.
- Zombies are likely former humans that became undead.
- The clothing of the majority of humans are reminiscent of Arabian fairy tales. There are some exceptions, such as Bolo and Mimic.