Featured in the Pirate Queen's Quest Half-Genie Hero DLC, Shantae will fight off against Risky Boots as the final boss. Her fighting style as a boss draws direct inspiration from Nega-Shantae's boss battle in Risky's Revenge. After Risky Boots clears the "Risky's Hideout" platforming segment she'll order her Tinkerbats to activate the Dynamo to which the stage is raised to where the Dynamo is located. Before Risky Boots attempts to activate the Dynamo Shantae interrupts her actions by entering the scene. She later explains how her friends were able to revert the magical inversion process before fighting Risky Boots.
Shantae attacks using her forms used in her campaign excluding the Dryad, Tinkerbat (backer-exclusive), gem pot, and blobfish transformations. She dances before being able to transform into each where dancing does not freeze time unlike her campaign. Unlike most boss battles, Shantae's visual appearance will change after receiving a significant amount of damage to show bruises and exhaustion.
- Monkey - Shantae will use monkey bullet to dash around the stage at random, occasionally launching herself at Risky.
- Elephant - Shantae will launch upwards and smash down as a giant form of the elephant, she'll attack in the area indicated by smoke.
- Mermaid - Shantae will jump into the air and spin while she fires bubbles at Risky
- Crab - Two waterfalls will appear as Shantae jumps off of the stage to horizontally attack from each waterfall. Shantae's location can be observed by bubbles emerging from the waterfall.
- Harpy - Shantae will fly off of the stage to attack Risky by swooping down in an arc from left to right.
- Spider - Shantae will jump into the air and rain spider venom onto the stage/
- Bat - Shantae will fly around the stage while obscuring all visuals except the small area around her, Shantae will emit a screech sound effect if the player has entered the light radius.
- Mouse - Shantae quickly runs around the stage to temporarily appear back in her human form to damage Risky
Upon defeating Shantae once and for all Risky attempts to activate the Dynamo. To Risky's surprise her Tinkerbats did not correctly construct the machine which explodes causing her Hideout to be destroyed, sending her flying away. After failing to capture sequin land yet again Risky berates her Tinkerbats once more before the game cuts to credits.